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磷酸肌酸激酶来源兔肌
磷酸肌酸激酶来源兔肌
磷酸肌酸激酶来源兔肌
商品货号:MB2902
CAS 号:9001-15-4
英文名字:Creatine phosphokinase from rabbit muscle
质量标准:Type I,≥150 units/mg,sigma分装
  • 包装规格:
    规格 库存 发货时间
    500U [¥200.00]14 现货
  • 购买数量:
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    商品信息

    质检证书(coa)

    说明书下载

    磷酸肌酸激酶来源兔肌,Creatine Phosphokinase from rabbit muscle; Type I, salt-free, lyophilized powder, ≥150 units/mg protein
    基本信息:

    type

    Type I

    form

    salt-free, lyophilized powder

    solubility

    0.25 M glycyl-glycine, pH 7.4: soluble5.0 mg/mL, clear, colorless to slightly yellow

    foreign activity

    ATPase ≤0.01%

    Lactic dehydrogenase, hexokinase, myokinase and pyruvate kinase ≤0.001%

    shipped in

    加冰

    storage temp.

    −20°C

    Preparation Note
    Produces a clear, colorless to light yellow solution at 5 mg/mL in 0.25 M glycyl-glycine, pH 7.4

    Unit Definition

    One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C.

    Application

    Molecular Weight: ~81,000
    Creatine Phosphokinase is a dimer composed predominantly of the skeletal muscle derived homodimer (MM). CK also exists as a heterodimer (MB) particularly in the myocardium. CK derived from brain tissue consists mainly of the brain source homodimer (BB). The amino acid sequences of the M chain and B chains are about 80% homologous. From the sequence, the molecular weight of the M chain is 43,112.
    E1%(280)= 8.76
    pH Optimum: pH 8.8-9.0 for the forward reaction and pH 6.0-7.0 for the reverse reaction.
    CK is a cellular enzyme with a wide tissue distribution. Its physiological role is associated with ATP generation for contractile or transport systems. Increased levels of CK are associated with myocardial infarction, muscular dystrophy, hyperthyroidism, pulmonary infarction and cerebrovascular disease. Variations in relative isozyme distribution can provide additional information in the diagnosis of these conditions.
    Substrates: Creatine, N-ethylglycocyamine and glyocyamine have been shown to act as substrates for CK. CK is very specific for ATP/ADP.
    Inhibitors: ADP is a strong inhibitor of the forward reaction competing with ATP. Divalent cations such as Ca2+ (Ki=4.5 mM), Zn2+ and Cu2+ inhibit CK by competing with Mg2+. Other inhibitors include acetate, acetylsalicylic acid, adenosine, p-aminosalicylic acid, AMP, benzoic acid, bicarbonate, bromide, chloride, p-Chloromercuribenzoic acid, ethylene oxide, 2,4-fluorodinitrobenzene, iodide, malonic acid, NAD, nitrate, phosphate, pyrophosphate, salicylic acid, sulfate, sulfite, thyroxine, trichloroacetate, L-triiodothyroxine, L-triiodothyronine, and tripolyphosphate.
    The enzyme from Sigma has been used in the measurement of phosphocreatine in hippocampal neurons isolated from rat brains.

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